The parasitic wasp Diglyphus isaea belongs to the family Eulophidae. It parasitizes leafminer larvae in field and greenhouse crops. The parasitic wasp is an ectoparasitoid, meaning that it lays eggs at the vicinity of the host (second and third instar larvae of the leafminer). It is native to Europe, Northern Africa and Japan, widely distributed throughout the Holarctic Region; also known from Middle East, Orient, Southeast Asia and Australia.

Target Pests
Leaf-mining flies in the genus Liriomyza (Diptera: Agromyzidae) are the most important hosts of Diglyphus isaea. It attacks nine species of leafminers, including Liriomyza trifolii, L. bryoniae, L. huidobrensis, L. cicerina, L. congesta, L. pusilla, L. pusio, L. sativae and L. strigata.

Leafminers attack a large number of plants, but seems to favour those in the plant families Cucurbitaceae, Leguminosae and Solanaceae. Diglyphus isaea is most efficient in controlling Liriomyza spp. in crops such as tomato, eggplant, ornamentals and also in open field. Other crops known as hosts include bean, pepper, potato, squash, cucumber, beet, pea and watermelon.

Diglyphus isaea is a small wasp (2 mm long) black with a metallic green sheen bearing short antennas. Females are slightly bigger than males and can be recognized by a wide black-colored band on the back pair of legs while males have two black-colored small bands. Eggs are of elongate shape, transparent white-coloured and measure 3 x 0,1 mm.
The adult parasitoid performs especially well during the hot conditions of mid-summer (temperatures above 30°C). It can also feed on the body fluids of the leafminer larvae to obtain protein – an essential ingredient of its diet – to maintain egg production. This activity, known as predatory host feeding, kills the host, in addition to mortality inflicted by active parasitization.

BioDiglyphus – The Product

  • BioDiglyphus is packed in a container of 125 cc containing 250-1000 adults.
  • If necessary, BioDiglyphus can be stored for 24 hours at a temperature of 6-8°C from the minute of arrival until release. The product should never be frozen.
  • BioDiglyphus is compatible with selective conventional chemicals (according to the list of compatible chemicals provided by BioBee).


  • BioDiglyphus is applied while walking in between the plants, opening the container’s lid and letting the wasps fly out towards the plants.
  • Application of BioDiglyphus should be carried out either early in the morning or late in the afternoon, under relatively mild temperatures.
  • BioDiglyphus is shipped in isolated styrofoam boxes chilled with icepacks. This packaging must be kept intact till it reaches the end-user. When applied, the containers should be taken from the shipment package one by one and the parasitoids should be released immediately.
  • The number of wasps at first introduction, as well as following introductions, depends upon the degree of leafminer infestation and the rate of naturally occurring parasitism.
  • About 3 weeks following the release of the parasitoids (depending upon temperature), a clear indication of active parasitism is visible. The subsequent established generations of the parasitic wasp are expected to effectively control the leaf miner throughout the entire period of the crop.



All products are tested to meet specification requirements before leaving the factory.
BioBee is not responsible for the outcome of implementation in the field, as it has no control over the method of application, local conditions, treatment/storage of product not according to instructions, etc.